Journal: eneuro

Article Title: Dorsal Medial Habenula Regulation of Mood-Related Behaviors and Primary Reinforcement by Tachykinin-Expressing Habenula Neurons

doi: 10.1523/eneuro.0109-16.2016

Figure Lengend Snippet: Figure 5. Specific expression of channelrhodopsin in tachykinin-expressing neurons in dMHb-TacChR2 mice. A, B, Comparison of Tac1 and Tac2 mRNA expression in the habenula; the axial level is approximately bregma 1.6mm. Data are derived from the Allen Mouse Brain Atlas. C, D, Conditional expression of ChR2-eYFP in Ai32 mice driven by Tac2IRESCre in the habenula. Dashed lines demarcate the extent of the habenula based on the expression of the nuclear factor Pou4f1 (C). Expression is infrequently seen in the vMHb, as defined by the expression of Chat (D). E, Conditional expression of tdTomato mRNA in Ai14 mice driven by Tac2IRESCre in the habenula (Allen Transgenic Mouse Characterization Project). F–H, ChR2-eYFP labeled fibers terminate predominantly in the lateral part of the interpeduncular nucleus, which receives afferents from the dMHb (F, H); these fibers are largely excluded from the IPR/IPC, which receive fibers mainly from the vMHb (G). I, J, Colocalization of SP, product of the Tac1 gene, with ChR2-eYFP in the interpeduncular nucleus of dMHb-TacChR2 mice. K, Colocalization of neurokinin B, product of the Tac2 gene, with ChR2-eYFP. Scale bars: A, 200 m; C, F, I, 100 m; J, 50 m.

Article Snippet: Mice for optogenetic studies of habenula function were generated by using the mouse line Ai32, which conditionally expresses the Channelrhodopsin-2 variant ChR2(H134R)YFP from a modified floxed-stop Gt(Rosa)26Sor locus (Madisen et al., 2012; RRID:IMSR_JAX:024109).

Techniques: Expressing, Comparison, Derivative Assay, Transgenic Assay, Labeling

Journal: Cell reports

Article Title: The Synaptic Organization of Layer 6 Circuits Reveals Inhibition as a Major Output of a Neocortical Sublamina

doi: 10.1016/j.celrep.2019.08.048

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse: loxP-STOP-loxP-ChR2-YFP Cre reporter lines (Ai32, Allen Institute for Brain Science) ( Madisen et al., 2012 ) , N/A , Jackson 012569.

Techniques: Virus, Plasmid Preparation, Recombinant, Electron Microscopy, Mutagenesis, Transgenic Assay, Software

Journal: The Journal of Neuroscience

Article Title: TRPV1-Lineage Somatosensory Fibers Communicate with Taste Neurons in the Mouse Parabrachial Nucleus

doi: 10.1523/JNEUROSCI.0927-21.2021

Figure Lengend Snippet: ChR2 expression was controlled by Cre recombinase in TRPV1-lineage afferents. A , Example electrophysiological recordings of Vc activity in one TRPV1-ChR2 mouse. Laser pulses applied to the medullary surface above the orosensory Vc reliably stimulated multiunit activity. Responses on individual trials and the overlay of responses across 18 trials, with one example in red, are shown. Note Vc recording was not part of the present analyses but is shown here to illustrate the photoexcitatory effect. B , Electrophysiological recordings of Vc unit activity in an Ai32 mouse. These mice express ChR2/EYFP only on exposure to Cre recombinase. Laser pulses applied to the medullary surface above the orosensory Vc did not excite Vc neurons in this mouse line, agreeing that ChR2 expression was not leaky. Overlays of 20 trials recorded from two example Vc neurons are shown. C , Microscope image of a coronal brain section showing GFP labeling of TRPV1-lineage processes in the spinal trigeminal tract (sp5) of TRPV1 Cre ;mT/mG mice. These mice provided positive control for immunohistochemical procedures performed on tissues from Ai32 mice. D , Cy5-labeled antibody staining against EYFP/GFP in panel C . E , DAPI labeling of cell nuclei in the section in C , D . F , Coronal section showing no EYFP labeling of fibers in the sp5 of Cre-negative Ai32 mice. G , Cy5-labeled antibody staining against EYFP/GFP applied the section in F yields no labeling, confirming ChR2/EYFP was not expressed. H , DAPI labeling showing cell nuclei in the section in F , G .

Article Snippet: TRPV1-ChR2 mice were the F1 progeny of a cross between homozygous TRPV1 Cre mice, which express Cre recombinase in TRPV1-lineage neurons (JAX #017769; Cavanaugh et al., 2011b ), and homozygous Ai32 mice, which direct Cre-dependent transcription of a ChR2/EYFP fusion gene (JAX #024109; Madisen et al., 2012 ).

Techniques: Expressing, Activity Assay, Microscopy, Labeling, Positive Control, Immunohistochemical staining, Staining